Construction of gene interruptions and gene deletions in the cyanobacterium Synechocystis sp. strain PCC 6803.

Genetic Manipulation of the Cyanobacterium Synechocystis sp . PCC 6803 ' Development of Strains Lacking Photosystem I for the Analysis of Mutations in

We have taken a genetic approach to eliminating the presence of photosystem I (PSI) in site-directed mutants of photosystem I I (PSII) in the cyanobacterium Synechocystis sp. PCC 6803. By selecting under light-activated heterotrophic conditions, we have inactivated the psaA-psaS operon encoding the PSI reaction center proteins in cells containing deletions of the three psbA genes. We have also ...

Circadian rhythm of the cyanobacterium Synechocystis sp. strain PCC 6803 in the dark.

The cyanobacterium Synechocystis sp. strain PCC 6803 exhibited circadian rhythms in complete darkness. To monitor a circadian rhythm of the Synechocystis cells in darkness, we introduced a PdnaK1::luxAB gene fusion (S. Aoki, T. Kondo, and M. Ishiura, J. Bacteriol. 177:5606-5611, 1995), which was composed of a promoter region of the Synechocystis dnaK1 gene and a promoterless bacterial luciferas...

Orus Synechocystis sp . strain PCC 6803 . formation in the cyanobacterium A gene ( ccmA ) required for carboxysome

A single vector-based strategy for marker-less gene replacement in Synechocystis sp. PCC 6803

BACKGROUND The cyanobacterium Synechocystis sp. PCC 6803 is widely used for research on photosynthesis and circadian rhythms, and also finds application in sustainable biotechnologies. Synechocystis is naturally transformable and undergoes homologous recombination, which enables the development of a variety of tools for genetic and genomic manipulations. To generate multiple gene deletions and/...

Four novel genes required for optimal photoautotrophic growth of the cyanobacterium Synechocystis sp. strain PCC 6803 identified by in vitro transposon mutagenesis.

Four novel Synechocystis sp. strain PCC 6803 genes (sll1495, sll0804, slr1306, and slr1125) which encode hypothetical proteins were determined by transposon mutagenesis to be required for optimal photoautotrophic growth. Mutations were also recovered in ccmK4, a carboxysome coat protein homologue, and me, the decarboxylating NADP(+)-dependent malic enzyme. This is the first report that these kn...